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Pretreatment with heat does not affect double-strand breaks DNA rejoining in Chlamydomonas reinhardtii |
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| Authors: | Stephka G Chankova Nadezhda P Yurina Evgeniya G Dimova Olga V Ermohina Yulia P Oleskina Maria T Dimitrova Peter E Bryant |
| Institution: | aCentral Laboratory of General Ecology, Bulgarian Academy of Sciences, 2 Gagarin Street, Sofia 1113, Bulgaria;bA.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky Prospect 33, 117071 Moscow, Russia;cBute Medical School, Bute Building, University of St Andrews, St Andrews, Fife, KY16 9TS, Scotland, UK |
| Abstract: | We aimed to clarify if heat pretreatment could protect Chlamydomonas reinhardtii cells from gamma rays DNA damaging action. It was studied whether: (1) heat pretreatment could accelerate DNA DSB rejoining; (2) chloroplast chaperones (HSP70B, HSP90C) could be involved in protection from radiation-induced DNA DSB.It was obtained that heat pretreatment (37–42 °C) induced minor DNA DSB levels which might be insufficient as signals for DNA DSB repair induction. No correlation between chaperones overproduction and DNA DSB rejoining was shown. These are probably the first data that HSP70B and HSP90C do not protect DNA against radiation-induced damage in a plant model system. |
| Keywords: | Adaptive response Chlamydomonas DNA DSB rejoining Gamma rays Heat-shock proteins |
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