Influence of medium and cold pretreatment on androgenetic response in Lolium perenne L. |
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Authors: | Hilde-Gunn Opsahl-Ferstad Åsmund Bjørnstad Odd Arne Rognli |
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Affiliation: | (1) Department of Biotechnological Sciences, Agricultural University of Norway, POB 5040, N-1432 Ås, Norway;(2) Department of Horticulture and Crop Sciences, Agricultural University of Norway, POB 5022, N-1432 Ås, Norway |
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Abstract: | Summary Genotypes of Lolium perenne L. with different androgenetic responses were used to test effects of induction medium composition. The media tested were potato II (pII), 190-2, and modified Linsmaier and Skoog media, LS-1, LS-2, and LS-3. The effect of different gelling agents, activated charcoal in a double layer design, and casein hydrolysate were also studied. From 36,696 anthers, 25,906 embryo-like structures, 1,959 albino and 173 green plants were generated. Significant differences were found between media, genotypes and medium-genotype interactions studied. All three media commonly used, pII, 190-2, and LS-3, were equivalent in production of green plants. Cold pretreatment of the anthers (4°C) significantly increased the number of embryo-like structures, the number and proportion of albino plants produced, but not the production of green plants.Abbreviations ELS embryo-like structures - ALB albino plants - ANT anthers - GRP green plants - DH doubled haploid plants - AC activated charcoal - CH casein hydrolysate - 2,4-D 2,4-dichlorophenoxyacetic acid - LS Linsmaier and Skoog (1965) basal medium - MS Murashige and Skoog (1962) - PL plants - pII potato II induction medium - DL double layer |
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