Simultaneous Chiral Separation of Flavanone,Naringenin, and Hesperetin Enantiomers by RP‐UHPLC‐DAD |
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Authors: | Irena Baranowska Judyta Hejniak Sylwia Magiera |
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Institution: | Department of Inorganic, Analytical Chemistry and Electrochemistry, Faculty of Chemistry, Silesian University of Technology, Gliwice, Poland |
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Abstract: | A rapid and effective RP‐UHPLC‐DAD method for enantioseparation of three flavanones, i.e., flavanone, naringenin, and hesperetin, was developed and validated. Chromatographic separation of the analytes was performed using a Chiralpak AD‐3R analytical column under reverse phase conditions with methanol as the mobile phase. The method was validated in the concentration range of 0.2 to 50 µg/mL for enantiomers of flavanone and 0.5 to 50 µg/mL for enantiomers of naringenin and hesperetin. The limits of quantification were between 0.03 to 0.5 µg/mL. Intraday and interday precision were below 14% and accuracy varied from 0.04 to 8.17%. Chirality 28:147–152, 2016. © 2015 Wiley Periodicals, Inc. |
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Keywords: | flavonoids enantiomers chromatography |
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