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Cross‐species amplification of microsatellite loci in aphids: assessment and application
Authors:Alex C C Wilson  Blandine Massonnet  Jean‐Christophe Simon  Nathalie Prunier‐Leterme  Lotfali Dolatti  Kate S Llewellyn  Christian C Figueroa  Claudio C Ramirez  Roger L Blackman  Arnaud Estoup  Paul Sunnucks
Abstract:Despite the relative ease of isolating microsatellites, their development still requires substantial inputs of time, money and expertise. For this reason there is considerable interest in using existing microsatellites on species from which markers were not cloned. We tested cross‐species amplification of 48 existing aphid loci in species of the following genera: Aphidinae: Aphidini: Aphis and Rhopalosiphum; Aphidinae: Macrosiphini: Acyrthosiphum, Brevicoryne, Diuraphis, Illinoia, Macrosiphoniella, Macrosiphum, Metopeurum, Metapolophium, Myzus, Phorodon, Sitobion and Uroleucon and Neuquenaphidinae: Neuquenaphis. Our results show cross‐species application of known microsatellite loci is a highly promising source of codominant markers for population genetic and evolutionary studies in aphids.
Keywords:Aphidini  cross‐priming  Macrosiphini  microsatellite  Neuquenaphidinae  X‐linked
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