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Isolation and characterization of polymorphic microsatellite loci from the Dungeness crab Cancer magister
Authors:Robert J Toonen  Melissa Locke  Rick Grosberg
Abstract:The Dungeness crab, Cancer magister, is the focus of one of the most intensely harvested fisheries in North America. Given its economic importance, there is considerable interest in assessing the degree and spatial pattern of genetic structure in C. magister. To that end, we developed a series of 17 hypervariable microsatellite loci. Six of these 17 loci could be amplified in a single multiplex PCR reaction. Using dye‐labelled primers all six loci can be coamplified and scored simultaneously on an automated sequencer. The ability to multiplex multiple loci greatly increases the ease and speed of genotyping for this species.
Keywords:Brachyura  Cancer magister  Dungeness crab  microsatellite  multiplex PCR
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