Cardioinhibitory peptides from Limulus polyphemus: modulation of the neurogenic heart |
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| Authors: | G Gaus M Casaretto H Stieve |
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| Institution: | (1) Institut für Biologie II (Zoologie), Kopernikusstr. 16, D-52056 Aachen, Germany;(2) Deutsches Wollforschungsinstitut, Veltmanplatz 8, D-52062 Aachen, Germany |
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| Abstract: | Four neuropeptides have been isolated and sequenced from acetone extracts of brains of the horseshoe crab Limulus polyphemus. They belong to a newly discovered peptide family in invertebrates. A possible role of the four peptides from Limulus as cardioregulatory neurotransmitters has been tested on the isolated Limulus heart. Three of the peptides (DEGHKMLYFamide, GHSLLHFamide, and PDHHMMYFamide) produce dose-dependent decreases in both amplitude and rate of the heart contractions, whereas DHGNMLYFamide reduces only the amplitude of the heartbeat. All four peptides differ in threshold, potency and duration of their effects.Abbreviations DIC
diisopropylcarbodiimide
- DTT
dithiotreitol
- Fmoc
9-fluorenylmethyloxycarbonyl
- GABA
gamma amino butyric acid
- HOBt
N-hydroxybenzotriazole
- HPLC
high performance liquid chromatography
- IBMX
3-isobutyl 1-methyl-xanthine
- Lip-HP
Limulus head peptide
- LP
Limulus peptide
- TBTU
2-(1H-benzotriazole-1yl)-1,1,3,3,tetramethyluronium tetrafluoroborate
- TFA
trifluoroacetic acid
- TLC
thin-layer chromatography |
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| Keywords: | Neuropeptides Cardioregulation Peptide synthesis Neurogenic heart Limulus polyphemus |
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