Observations on sporozoite detection in naturally infected sibling species of the <Emphasis Type="Italic">Anopheles culicifacies</Emphasis> complex and variant of <Emphasis Type="Italic">Anopheles stephensi</Emphasis> in India |
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Authors: | Susanta Kumar Ghosh Satyanarayan Tiwari Kamaraju Raghavendra Tiruchinapalli Sundaraj Sathyanarayan Aditya Prasad Dash |
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Institution: | (1) National Institute of Malaria Research (ICMR), Epidemic Diseases Hospital, Old Madras Road, Bangalore, 560 038, India;(2) National Institute of Malaria Research (ICMR), 22 Sham Nath Marg, Delhi, 110 054, India |
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Abstract: | Sporozoites were detected in naturally infected sibling species of the primary rural vector Anopheles culicifacies complex in two primary health centres (PHCs) and a variant of the urban vector Anopheles stephensi in Mangalore city, Karnataka, south India while carrying out malaria outbreak investigations from 1998–2006. Sibling species
of An. culicifacies were identified based on the banding patterns on ovarian polytene chromosomes, and variants of An. stephensi were identified based on the number of ridges on the egg floats. Sporozoites were detected in the salivary glands by the
dissection method. Of the total 334 salivary glands of An. culicifacies dissected, 17 (5.08%) were found to be positive for sporozoites. Of the 17 positive samples, 11 were suitable for sibling
species analysis; 10 were species A (an efficient vector) and 1 was species B (a poor vector). Out of 46 An. stephensi dissected, one was sporozoite positive and belonged to the type form (an efficient vector). In malaria epidemiology this
observation is useful for planning an effective vector control programme, because each sibling species/variant differs in
host specificity, susceptibility to malarial parasites, breeding habitats and response to insecticides. |
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Keywords: | An culicifacies sibling species An stephensi variants malaria sporozoites |
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