The detection of the sialoglycoprotease gene and assay for sialoglycoprotease activity among isolates of Pasteurella haemolytica A1 strains, serotypes A13, A14, T15 and A16 |
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| Authors: | Chiang W. Lee Reggie Y.C. Lo Patricia E. Shewen Alan Mellors |
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| Affiliation: | Department of Veterinary Microbiology and Immunology, University of Guelph, Guelph, Ontario N1G 2W1, Canada; Department of Microbiology, University of Guelph, Guelph, Ontario N1G 2W1, Canada; Department of Chemistry and Biochemistry, University of Guelph, Guelph, Ontario N1G 2W1, Canada |
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| Abstract: | Abstract Polymerase chain reaction (PCR) using specific primers to the sialoglycoprotease gene ( gcp ) of Pasteurella haemolytica biotype A, serotype 1 amplified a 1-kb fragment from each of P. haemolytica serotypes A7, A13, A14 and A16, but not T15; which was confirmed by Southern blot hybridization analysis. Using a sialoglycoprotease (Gcp) activity assay, Gcp activity was found in serotypes A13, A14 and A16. Inclusion of these three serotypes confirms that all recognized A biotypes are positive for both gcp gene and activity, with the exception of serotype A11 (which has a different genetic organization and exhibits no Gcp activity). Furthermore, all recognized T biotypes are negative for both the gene and Gcp activity. |
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| Keywords: | Pasteurella haemolytica A1 Biotype A Biotype T Sialoglycoprotease |
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