Influence of vector design and host cell on the mechanism of recombination and emergence of mutant subpopulations of replicating retroviral vectors |
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| Authors: | Matthias Paar Dieter Klein Brian Salmons Walter H Günzburg Matthias Renner Daniel Portsmouth |
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| Institution: | (1) Institute of Virology, Department of Pathobiology, University of Veterinary Medicine, Vienna, Austria;(2) Christian-Doppler Laboratory for Gene Therapeutic Vector Development, Vienna, Austria;(3) Vetomics – Core Facility for Research, University of Veterinary Medicine, Vienna, Austria;(4) Austrianova Singapore Pte Ltd, 20 Biopolis Way, #05-518 Centros, Singapore, 138668, Singapore |
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| Abstract: | Background The recent advent of murine leukaemia virus (MLV)-based replication-competent retroviral (RCR) vector technology has provided exciting new tools for gene delivery, albeit
the advances in vector efficiency which have been realized are also accompanied by a set of fresh challenges. The expression
of additional transgene sequences, for example, increases the length of the viral genome, which can lead to reductions in
replication efficiency and in turn to vector genome instability. This necessitates efforts to analyse the rate and mechanism
of recombinant emergence during the replication of such vectors to provide data which should contribute to improvements in
RCR vector design. |
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