High frequency plant regeneration from the mature seeds of Garcinia indica

A high frequency plant regeneration system was developed for the production of high yielding elite clones of Garcinia indica via direct organogenesis. A maximum number of 86.2 shoot buds per explant were induced from the mature seed segments cultured on Woody plant medium (WPM) supplemented with 2.0 mg dm⁻³ N⁶-benzyladenine and 1 mg dm⁻³ indole-3-acetic acid. Rooting was achieved on half-strength WPM medium supplemented with 3 mg dm⁻³ indole-3-butyric acid. Rooted plantlets were acclimatized and transferred to greenhouse for further growth. The highest survival rate of 95 % was recorded using a mixture of garden soil and sand. Histological studies clearly revealed multiple shoot formation from mature seed segments.