Isolation of lipopolysaccharides and the effect of Polymyxin B on the outer membrane of Corynebacterium autotrophicum |
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Authors: | Jürgen Wiegel Frank Mayer |
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Institution: | (1) Institut für Mikrobiologie, Abteilung für Bakterienphysiologie, Gesellschaft für Strahlen- und Umweltforschung mbH, Göttingen, Federal Republic of Germany;(2) Institut für Mikrobiologie der Universität Göttingen, Grisebachstr. 8, D-3400 Göttingen, Federal Republic of Germany;(3) Present address: Department of Biochemistry, University of Georgia, 30602 Athens, GA, USA |
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Abstract: | Lipopolysaccharides (LPS) from Corynebacterium autotrophicum were isolated and analyzed. Autotrophically grown cells contained 2–5 mg of partly purified LPS per g dry weight of lyophilized cells. Serological cross reaction with Lipid A antigen of Salmonella minnesota confirmed the presence of LPS in C. autotrophicum. Electron microscopy of negatively stained Polymyxin B-treated cells showed formation of blebs on the Outer Membrane indicating an interaction of Polymyxin B specifically with LPS. Up to now, no Gram-positive organisms are known which contain any LPS. Thus, C. autotrophicum, though giving opposite results when the Gram-staining reaction was applied by several authors, has to be classified into the group of Gram-negative bacteria.Non-Common Abbreviations LPS
lipopolysaccharide
- KDO
2-keto-3-deoxyoctonate |
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Keywords: | Corynebacterium autotrophicum Outer Membrane Lipopolysaccharides Biochemical analysis Polymyxin B Electron microscopy |
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