Molecular Characterization and <Emphasis Type="Italic">in planta</Emphasis> Detection of <Emphasis Type="Italic">Sclerotinia sclerotiorum</Emphasis> Endopolygalacturonase Genes |
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Authors: | Zsolt Kasza Csaba Vagvölgyi Michel Févre Pascale Cotton |
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Institution: | (1) Department of Microbiology, Faculty of Sciences, University of Szeged, PO Box 533, 6701 Szeged, Hungary;(2) Laboratoire de Biologie Cellulaire Fongique, UMR 5122 CNRS INSA UCB, Bat Lwoff, 10 rue Dubois, Université C. Bernard Lyon1, 69622 Villeurbanne cedex, France |
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Abstract: | Sclerotinia sclerotiorum, a plant pathogenic ascomycete, secretes multiple pectinolytic enzymes that facilitate penetration, colonization, and maceration of the plant tissues. Molecular analysis has previously revealed that the pectinolytic system of the fungus is organized as a multigene family, among which a subfamily of three members encoding for neutral endopolygalacturonase (endoPG) isoforms has been characterized. Here we describe the isolation and characterization of three additional endoPG-encoding genes (pg5, pg6, and pg7) that belong to distinct phylogenetic groups. Pairwise sequence comparison between the known endoPGs from S. sclerotiorum revealed 43% to 97% identity, and the genomic organization of the pectinolytic system showed a great similarity to that of the related necrotroph Botrytis cinerea. During plant pathogenesis, a sequential expression of the endoPG-encoding genes was shown. |
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