Plant Nucleases: III. Polyacrylamide Gel Electrophoresis of Corn Ribonuclease Isoenzymes |
| |
Authors: | Wilson C M |
| |
Affiliation: | Crops Research Division, Agricultural Research Service, United States Department of Agriculture, and Department of Agronomy, University of Illinois, Urbana, Illinois 61801. |
| |
Abstract: | Isoenzymes of RNase were detected in plant extracts after polyacrylamide gel electrophoresis with a new buffer system. The gels were incubated in an RNA solution, then dipped for 30 seconds into 0.2% toluidine blue. The method is rapid and is sensitive to very small amounts of RNase. The effects of buffers and ethylenediaminetetraacetate on the different enzymes are illustrated by photographs and scans of the gels. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|