Utilization of an auxotrophic strain of the yeast Yarrowia lipolytica to improve γ-decalactone production yields |
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Authors: | Y Pagot A Endrizzi J-M Nicaud & J-M Belin |
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Institution: | Unitéde Recherches de Biologie Moléculaire, CNEVA Ploufragan, Zoopôle Les Croix, Ploufragan,;Unitéde Recherches en Qualitéet Sécuritédes Aliments, CNEVA Ploufragan, Zoopôle Beaucemaine, Ploufragan, France |
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Abstract: | Here, hybridization assay of amplified products is described which detect Salmonella sp. from chicken fillets and other food homogenates within 24 h. This technique is composed of four steps : (1) sample is pre-enriched overnight in phosphate buffered peptone water ; (2) total DNA is extracted ; (3) a Salmonella spp. specific DNA target sequence is amplified by polymerase chain reaction ; (4) amplified products are captured by a probe covalently bound onto NH-CovalinkTM (Nunc, Danemark) microwells and detected by a chemiluminescent enzymatic reaction. This hybridization of amplified products was demonstrated as sensitive as their analysis on agarose gel. Compared to a bacteriological method for Salmonella spp. detection, its specificity was estimated at 100% and its sensitivity was 93·2% from analysis of 207 naturally contaminated chicken fillets samples. |
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