Isolation of additional monoclonal antibodies directed against cell surface antigens of Myxococcus xanthus cells undergoing submerged development. |
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| Authors: | J S Gill and M Dworkin |
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| Affiliation: | Department of Microbiology, University of Minnesota, Minneapolis 55455. |
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| Abstract: | Thirteen additional monoclonal antibodies directed against cell surface antigens of Myxococcus xanthus cells undergoing submerged development were isolated and partially characterized. As measured by quantitative enzyme-linked immunosorbent assay, 10 of these antibodies recognized antigens common to both vegetatively growing cells and cells undergoing submerged development; 3 antibodies recognized antigens specific to developing cells. Five antigens were revealed as single bands on Western blots (immunoblots), and one produced multiple, diffuse bands characteristic of lipopolysaccharide. |
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