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Detection of virulent Shigella and enteroinvasive Escherichia coli by induction of the 43 kDa invasion plasmid antigen, ipaC |
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| Authors: | Rita Shamlal Shantha Rajarathnam Krishnan Sankaran Vasanthi Ramachandran Yerramilli VBK Subrahmanyam GBalakrish Nair Ragini Macaden Raman K Roy |
| Institution: | Astra Research Centre India, P.B. No. 359, 18th Cross Malleswaram, Bangalore 560 003, India;St. John's Medical College, Department of Microbiology, Bangalore 560 034, India;National Institute of Cholera and Enteric Diseases, P-33, CIT Scheme XM, Beliaghata, Calcutta 700 010, India |
| Abstract: | The invasion plasmid antigen, ipaC (43 kDa) of Shigella spp. and enteroinvasive Escherichia coli (EIEC) could be induced in vitro by growing them in the presence of Congo red. An enzyme-linked immunosorbent assay (ELISA) using antibodies to the 43 kDa protein of Shigella has been developed for specific detection of virulent Shigella spp and EIEC. The test is independent of initial isolation of individual colonies. As few as 102 CFU/ml of virulent Shigella present in mixed cultures could be detected and concurrently their susceptibility to antibiotics could be analysed after an initial growth of 8–16 h in Congo red-containing medium. The test may prove useful in the diagnosis and treatment of bacillary dysentery caused either by Shigella or EIEC through their rapid identification and proper antimicrobial therapy. |
| Keywords: | Diagnosis Shigella Enteroinvasive Escherichia coli Induction ipaC |
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