利用花粉管通道技术培育番茄耐盐新种质

利用白花授粉后形成的花粉管通道分别将番茄耐盐野生近缘种Lycopersicon peruvianum LAlll、Lycopersicon cheesmanii LAl66、Lycopersicon pennellii LA716、Lycopersicon pimpinellifolium LA2184的总DNA及含来源于大麦LEA基因家族的HVAl基因的pBY520质粒DNA导人栽培番茄“鲜丰”及“矮黄”，获得了较为广泛的变异，经过对后代的选择培育获得了一批农艺性状优良的耐盐新种质，并已培育耐盐新品系1个；传统的叶色遗传与现代的PCR检测表明番茄通过花粉管通道导人外源DNA是可行的。

Developing new germplasms of Lycopersicon esculentum for salt tolerance by pollen tube method

Via the pollen tubes formed after self-pollination,the total DNA of wild salt-tolerant tomato species Lycopersicon peruvianum LA111,Lycopersicon cheesmanii LA166,Lycopersicon pennellii LA716,Lycopersicon pimpinellifolium LA2184 and pBY520 containing HVA1 gene,which is one of the members of LEA (Late Embryogenesis Abundant) protein family,played an important role in plant survival under stress such as salt and drought,were introduced into tomato cultivar 'xianfeng' and 'aihuang'.There was obvious improvement in the salt tolerance among the transgenic plants,a number of new salt-tolerant germplasms were developed and a new line was bred through selection and identification,PCR test also demonstrated that it is feasible to introduce exogenous DNA through pollen tubes.