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Onset of neurophysin self-association upon neurophysin/neuropeptide hormone precursor biosynthesis |
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| Authors: | I M Chaiken H Tamaoki M J Brownstein H Gainer |
| Institution: | 1. Laboratory of Chemical Biology, National Institute of Arthritis, Building 10, Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20205, USA;2. Laboratory of Neurochemistry and Neuroimmunology, National Institute of Child Health and Human Development, Bethesda, MD 20205, USA;3. Laboratory of Cell Biology, National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20205, USA |
| Abstract: | 32P-Labelled washed rabbit platelets were incubated with 0.6 nM platelet activating factor (PAF-acether), giving a full aggregation and release response within 30-60 s. The major phospholipid changes observed under these conditions were: (1) An increased labelling of phosphatidic acid (PA) within 10 s and of phosphatidylinositol (MPI) at 30 s, reflecting the activation of the MPI cycle via the cytosolic phospholipase C; (2) an enhancement of phosphatidylinositol-4-phosphate (DPI) and phosphatidylinositol-4,5-bisphosphate (TPI) labelling at later incubation times; (3) an early degradation of TPI with a counterbalancing formation of DPI. The latter changes suggest a receptor-mediated stimulation of TPI-phosphomonoesterase, the role of which in the mechanism of platelet activation is discussed. |
| Keywords: | Neuropeptide hormone Neurophysin Prohormone association Hormone—protein interaction Quantitative affinity chromatography BNP-II bovine neurophysin II (vasopressin-associated) NP neurophysin LVP and AVP lysine and arginine vasopressin OT oxytocin pro-NP/AVP pulse-labelled biosynthetic precursor for AVP and AVP-associated NP pro-NP/OT pulse-labelled biosynthetic precursor for OT and OT-associated NP |
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