Assignment of anonymous DNA probes to specific intervals of human chromosomes 16 and X |
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Authors: | V. J. Hyland K. E. W. Fernandez D. F. Callen R. N. MacKinnon E. Baker K. Friend G. R. Sutherland |
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Affiliation: | (1) Cytogenetics Unit, Department of Histopathology, Adelaide Children's Hospital, 5006 North Adelaide, S. A., Australia |
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Abstract: | Summary Anonymous DNA probes mapping to human chromosome 16 and the distal region of the human X chromosome were isolated from a genomic library constructed using lambda EMBL3 and DNA from a mouse/human hybrid. The hybrid cell contained a der(16)t(X;16)(q26;q24) as the only human chromosome. Fifty clones were isolated using total human DNA as a hybridisation probe. Forty six clones contained single copy DNA in addition to the repetitive DNA. Pre-reassociation with sonicated human DNA was used to map these clones by a combination of Southern blot analysis of a hybrid cell panel containing fragments of chromosomes 16 and X and in situ hybridisation. One clone mapped to 16pter 16p13.11, one clone to 16p13.3 16p13.11, four clones to 16p13.3 16p13.13, two clones to 16p13.13 16p13.11, one clone to 16p13.11, seven clones to 16p13.11 16q12 or 16q13, four clones to 16q12 or 16q13, three clones to 16q13 16q22.1, four clones to 16q22.105 16q24, and nineteen clones to Xq26 Xqter. Two clones mapping to 16p13 detected RFLPs. VK5 (D16S94) detected an MspI RFLP, PIC 0.37. VK20 (D16S96) detected a TaqI RFLP, PIC 0.37 and two MspI RFLPs, PIC 0.30 and 0.50. The adult polycystic kidney disease locus (PKD1) has also been assigned to 16p13. The RFLPs described will be of use for genetic counselling and in the isolation of the PKD1 gene. Similarly, the X clones may be used to isolate RFLPs for genetic counselling and the isolation of genes for the many diseases that map to Xq26 qter. |
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