Comparison of PCR-RFLP analysis of the ITS region with morphological criteria of various strains of Dunaliella |
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Authors: | Mariela A González Patricia I Gómez Rolando Montoya |
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Institution: | (1) Departamento de Botánica, Facultad de Ciencias Naturales y Oceanográficas, Chile;(2) Departamento de Biología Molecular, Facultad de Ciencias Biológicas, Universidad de Concepción, Casilla 2407, Concepción, Chile |
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Abstract: | The genus Dunaliella comprises 28 species defined primarily by morphological and physiological criteria, which vary considerably
depending on growth conditions. Concomitantly, the taxonomic status of various species is uncertain. To confirm the taxonomic
identity and to better understand the relationship within Dunaliella, seven taxa ( D. salina, D. bardawil, D. tertiolecta,
D. parva, D. viridis, D. lateralis, D. peircei) were compared using RFLP analysis of the nuclear rDNA repeats, specifically
the internal transcribed spacer regions, including the 5.8S rRNA gene. Volvox aureus was used as an outgroup. A single ITS
PCR amplification product was obtained for each taxon. An ITS fragment of ca. 640 bp was present in all the taxa within the
subgenus Dunaliella, except for D. salina CCMP 1303 (ca. 540 bp) and D. lateralis (subgenus Pascheria) (ca. 600 bp). A cluster
analysis based on the presence or absence of bands generated by digestion of the PCR product with 8 restriction endonucleases
(DpnI, HhaI, EcoRI, PvuII, TaqI, HaeIII, MspI, StyI) revealed no correlation between the genetic relationship inferred from
the ITS-RFLP data and the morpho-physiological attributes used for taxonomy. In addition, differences in morphology, physiology
and in the length and restriction fragment patterns of the ITS region of D. salina CCMP 1303 suggest that this strain does
not belong to Dunaliella.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | Dunaliella ITS PCR RFLP strains taxonomy |
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