Characterization of phi HAU3, a broad-host-range temperate streptomyces phage, and development of phasmids.

phi HAU3 is a temperate Streptomyces phage with cohesive ends and a broad host range that includes Streptomyces hygroscopicus 10-22, a producer of antifungal compounds, but it fails to grow on Streptomyces lividans 66. Two phasmid derivatives were constructed that function as lambda cosmid vectors in Escherichia coli and as phages in Streptomyces spp.