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Acidic glycosphingolipids of cock testis elucidated by mass spectrometry and NMR spectroscopy
Authors:Dumitru-Petru Iga  Silvia Iga  Thomas Larsson  Jonas Ångström  Bassam Soussi  Olivier Rakotonirainy  Halina Miller-Podraza
Institution:(1) Faculty of Biology, Splaiul Independentei 95, s, R-76201, Roumania;(2) Institute of Medical Biochemistry, GÖteborg University, Göteborg University, Box 440, SE 405 30 Göteborg, Sweden;(3) Wallenberg Laboratory-NMR Lab, Sahlgrenrsquos University Hospital, S-413 45 Göteborg, Sweden
Abstract:The main acidic glycosphingolipids (GSLs) of cock testis were identified as GalCer I3-sulfate and gangliosides GM4, GM3, GD3 and GT3. They contained N-acetylneuraminic acid as the major sialic acid, and ceramides composed mainly of sphingosine (dl8:1) and C18–24 non-hydroxy fatty acids. Appreciable amounts of hydroxy fatty acids were detected only in the GM4 preparation.
Keywords:ganglioside  sufatide  cock testis
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