Crystallization and preliminary X-ray studies of pyridoxal kinase from sheep brain |
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| Authors: | A Arnone P Rogers G Scholz F Kwok |
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| Institution: | Department of Biochemistry, University of Iowa, Iowa City 52242. |
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| Abstract: | Crystals of pyridoxal kinase from sheep brain have been grown from solutions of ammonium sulfate or Na+/K+ phosphate. The crystals are trigonal, space group P3(1)21 with axes a = b = 102.2 A and c = 58.5 A. The crystals are quite stable to x-rays and diffract at 2.2-A resolution. This macromolecule is a 80,000-kDa dimer and utilizes the 2-fold symmetry of the space group, resulting in one subunit/asymmetric unit. |
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