| 北京棒状杆菌AS1.299谷氨酰胺合成酶活力调节的研究 |
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| 引用本文： | 钱世钧,孟广震,郝凤兮,姜维真.北京棒状杆菌AS1.299谷氨酰胺合成酶活力调节的研究[J].中国生物化学与分子生物学报,1986,2(2):43-50. |
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| 作者姓名： | 钱世钧 孟广震 郝凤兮 姜维真 |
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| 作者单位： | 中国科学院微生物研究所 北京
(钱世钧，孟广震，郝凤兮)，中国科学院微生物研究所 北京(姜维真) |
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| 摘 要： | 北京棒状杆菌(Corynebacterium pekinense AS1.299)谷氨酰胺合成酶的转移酶活性依赖于Mn~(++),酶的生物合成酶活性依赖于Mg~(++),其他二价金属离子只能部分代替Mn~(++)和Mg~(++)的作用。Mn~(++)对ATP或ADP的克分子比对酶活力起调节作用。ATP、CTP,丙氨酸和甘氨酸对谷氨酰胺合成酶有较强的抑制作用;丝氨酸、谷氨酸和6-磷酸葡萄糖胺对酶活力的抑制作用分别是24,15和21％。效应物混合物对酶的作用被证明是累积性的抑制作用。
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| 关 键 词： | 谷氨酰胺合成酶 北京棒状杆菌 调节作用 |
| 收稿时间： | 1986-04-20 |
STUDIES ON ACTIVITY REGULATION OF GLUTAMINE SYNTHETASE FROM CORYNEBACTERIUM PEKINENSE AS 1.299 |
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| Qian,Shi-jun Meng,Guang-zhen Hao,Feng-xi Jiang,wei-zhen.STUDIES ON ACTIVITY REGULATION OF GLUTAMINE SYNTHETASE FROM CORYNEBACTERIUM PEKINENSE AS 1.299[J].Chinese Journal of Biochemistry and Molecular Biology,1986,2(2):43-50. |
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| Authors: | Qian Shi-jun Meng Guang-zhen Hao Feng-xi Jiang wei-zhen |
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| Institution: | (Institute of Microbiology,Academia Sinica,Beijing |
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| Abstract: | The γ-glutamyl transferase and biosynthetic activities of glutamine synthetase from Corynebacterium pekinense AS 1.299 were dependent on Mn++ and Mg++ respectively,other divalent cation could only partially substitute for Mn++ or Mg++.The molar ratio of Mn4++ to ATP or ADP was important for glutamine synthetase activities.ATP,CTP,alanine and glycine exhibited obvious inhibition to transferase activity.Serine,glutamic acid and glucos-amine-6-phosphate inhibited transferase activity by 24,15 and 21% respctively.The combination effects of mixed inhibitors on glutamine synthetase activity were proved to be cumulative inhibition, |
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| Keywords: | Glutamine synthetase Corynebacterium pekinense Regulation |
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