Isolation and Characterization of a Cytokinin-Binding Protein from the Water-Soluble Fraction of Tobacco Leaves |
| |
Authors: | Momotani Eiki; Tsuji Hideo |
| |
Institution: | Department of Botany, Faculty of Science, Kyoto University Kyoto, 606-01 Japan |
| |
Abstract: | Cytokinin-binding protein (CBPI) was purified from the watersoluble fraction of tobacco leaves by successive chromatographyon benzyladenine-linked (BA-linked) Sepharose 4B, TSK-Gel G3000SWXL,t-zeatin-linked Sepharose 6B and TSK-Gel G3000SWXL. CBPI wasobtained as a monomer with a molecular weight of 31 kDa. Ithas one cytokinin-binding site, which shows a high affinityfor BA (Kd=1.1x107 M) and other cytokinins. Biologicallyactive cytokinins competed with BA for binding to this protein,while biologically inactive analogues of adenine did not. Inall cases, cytokinin-binding activity was assayed by equilibriumdialysis.
1 Present address: National Institute for Basic Biology, Okazaki,444 Japan. |
| |
Keywords: | |
本文献已被 Oxford 等数据库收录! |
|