Real-time observation of acrosomal dispersal from mouse sperm using GFP as a marker protein. |
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| Authors: | T Nakanishi M Ikawa S Yamada M Parvinen T Baba Y Nishimune M Okabe |
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| Institution: | Genome Information Research Center, Osaka University, Suita, Japan. |
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| Abstract: | We produced transgenic mouse lines that accumulate mutated green fluorescent protein (EGFP) in sperm acrosome, a membrane limited organelle overlying the nucleus. The sperm showed normal fertilizing ability and the integrity of their acrosome was easily examined in a non-invasive manner by tracing the GFP in individual 'live' sperm with fluorescent microscopy. The time required for the dispersal of acrosomal contents was demonstrated to be approximately 3 s after the onset of acrosome reaction. |
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