Retrograde regulation of nuclear gene expression in CW-CMS of rice |
| |
Authors: | Sota Fujii Setsuko Komatsu Kinya Toriyama |
| |
Institution: | (1) Laboratory of Environmental Biotechnology, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan;(2) Laboratory of Gene Regulation, Department of Molecular Genetics, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan |
| |
Abstract: | The CW-cytoplasmic male sterility (CMS) line has the cytoplasm of Oryza rufipogon Griff, and mature pollen is morphologically normal under an optical microscope but lacks the ability to germinate; restorer
gene Rf17 has been identified as restoring this ability. The difference between nuclear gene expression in mature anthers was compared
for the CW-CMS line, cms-CW] rf17rf17, and a maintainer line with normal cytoplasm of Oryza sativa L., normal] rf17rf17. Using a 22-k rice oligoarray we detected 58 genes that were up-regulated more than threefold in the CW-CMS line. Expression
in other organs was further investigated for 20 genes using RT-PCR. Five genes, including genes for alternative oxidase, were
found to be preferentially expressed in cms-CW] rf17rf17 but not in normal] rf17rf17 or cms-CW] Rf17Rf17. Such cms-CW] rf17rf17-specific gene expression was only observed in mature anthers but not in leaves, stems, or roots, indicating the presence
of anther-specific mitochondrial retrograde regulation of nuclear gene expression, and that Rf17 has a role in restoring the ectopic gene expression. We also used a proteomic approach to discover the retrograde regulated
proteins and identified six proteins that were accumulated differently. These results reveal organ-specific induced mitochondrial
retrograde pathways affecting nuclear gene expression possibly related to CMS.
Electronic Supplementary Material Supplementary material is available to authorised users in the online version of this article at . |
| |
Keywords: | Cytoplasmic male sterility ms-CW Oligoarray Oryza sativa L Oryza rufipogon Griff Proteome |
本文献已被 PubMed SpringerLink 等数据库收录! |
|