Evidence of Symbiosis Between the Soil Yeast <Emphasis Type="Italic">Cryptococcus laurentii</Emphasis> and a Sclerophyllous Medicinal Shrub, <Emphasis Type="Italic">Agathosma betulina</Emphasis> (Berg.) Pillans |
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Authors: | Karen?J?Cloete Alexander?J?Valentine Marietjie?A?Stander Louisa?M?Blomerus Email author" target="_blank">Alfred?BothaEmail author |
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Institution: | (1) Department of Microbiology, Faculty of Science, University of Stellenbosch, Private Bag X1, MATIELAND, 7602 Western Cape, South Africa;(2) Plant Biology Division, The Samuel Roberts Noble Foundation, Inc., P.O. Box 2180, Ardmore, OK 73401, USA;(3) LC-MS laboratory, J.C. Smuts building room 255, Faculty of Science, University of Stellenbosch, Private Bag X1, MATIELAND, 7602 Western Cape, South Africa;(4) Agricultural Research Council-Roodeplaat, Vegetable and Ornamental Plant Institute, Private Bag X5026, Stellenbosch, 7599, South Africa; |
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Abstract: | The interaction between a common soil yeast, Cryptococcus laurentii, and a slow-growing medicinal plant adapted to low-nutrient soils, Agathosma betulina (Berg.) Pillans, was studied. C. laurentii CAB 578 was isolated from the rhizosphere of wild A. betulina, and liquid chromatography-tandem mass spectrometry (LC-MS-MS) analysis revealed that the yeast was capable of producing
polyamines, such as cadaverine and spermine, while growing in vitro in a chemically defined medium. Since the exogenous application
of polyamines are known to impact on root growth, these findings supported the results obtained when axenic cultures of A. betulina seedlings were inoculated with C. laurentii CAB 578 and cultivated for 5 months under glasshouse conditions. The presence of the yeast increased root growth by 51%.
Using soil dilution plates, it was demonstrated that yeast numbers were greater in the vicinity of the roots than in the bulk
soil. In addition, fluoromicroscopy, in combination with the fluorescent probes Fungolight and Calcofluor white, revealed the presence of metabolic active yeast colonies on the rhizoplane 5 months after initiation
of the experimentation. The study provided evidence for a symbiosis between C. laurentii and A. betulina.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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