白介素-18在被动吸烟诱导的慢性阻塞性肺疾病大鼠中的表达

目的 研究白介紊-18(Interleukin-18,IL-18)在被动吸烟诱导的慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)大鼠中的表达变化.方法 将20只大鼠随机分为2组,即正常对照组和COPD模型组.应用单纯被动吸烟法建立大鼠COPD模型,香烟烟雾暴露时间为6个月.利用酶联免疫吸附法测定2组大鼠血清和支气管肺泡灌洗液(bronchoal veolar lavage fluid,BALF)中的IL-18浓度,用实时定量RT-PCR法测定BALF中IL-18 mRNA的表达水平,用HE染色法观察肺组织形态学改变,用免疫组织化学染色法检测IL-18在肺组织中的表达.结果 1.COPD模型组血清和BALF中的IL-18浓度较正常对照组显著增加(P＜0.01)；2.COPD模型组BALF中IL-18 mRNA的表达水平较正常对照组显著增高(P＜0.01)；3.COPD模型组肺组织中IL-18的表达较正常对照组显著增加(P＜0.01).结论 被动吸烟诱导的COPD大鼠外周血和肺部均高表达IL-18,提示IL-18在吸烟所致的COPD发病机制中可能起重要作用.

Expression of interleukin-18 in rats with induced by passive smoking chronic obstructive pulmonary disease

Objective To study the expression of interleukin-18(IL-18) in rats with passive smoking induced chronic obstructive pulmonary disease(COPD). Methods Twenty rats were randomly divided into a normal control group and a COPD model group.Rat COPD model was established by passive smoking for 6 months.The levels of IL-18 in serum and bronchoalveolar lavage fluid(BALF) were measured by ELISA.The levels of IL-18 mRNA in BALF were determined by real time RT-PCR.The lung tissue pathology was observed with HE staining.Immunohistochemical staining was used to investigate the expression of IL-18 in pulmonary tissues. Results 1.The levels of IL-18 in serum and BALF in the COPD group were significantly higher than those in the control group(P<0.01).2.The levels of IL-18 mRNA in BALF were significantly higher in the COPD group than in the control group(P<0.01).3.The expressions of IL-18 in pulmonary tissues were significantly higher in the COPD group than in the control group(P<0.01). Conclusion The expression of IL-18 significantly increased in peripheral blood and lung of rats with passive smoking induced COPD.Theses results suggest that IL-18 may be implicated in the pathogenesis of COPD caused by smoking.