Ochratoxin A induces apoptosis in neuronal cells |
| |
Authors: | Xiangnan Zhang Christine Boesch-Saadatmandi Yijia Lou Siegfried Wolffram Patricia Huebbe Gerald Rimbach |
| |
Institution: | 1Institute of Pharmacology, Toxicology and Biochemical Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, 310058 Hangzhou, China ;2Institute of Human Nutrition and Food Science, Christian Albrechts University Kiel, Hermann-Rodewald-Strasse 6, 24098 Kiel, Germany ;3Institute of Animal Nutrition and Physiology, Christian Albrechts University, Kiel, Germany |
| |
Abstract: | The mycotoxin ochratoxin A (OTA), which is produced by Aspergillus and Penicillium subspecies, is a frequently present contaminant of food and feedstuffs. OTA exhibits a wide range of toxic activities including nephro- and hepatotoxicity. However, little is known regarding potential neurotoxic effects of OTA. In the present study primary neurons as well as SH-SY5Y neuronal cells were incubated with increasing concentrations of OTA (0.1–2.5 μmol/L). OTA treatment resulted in a dose-dependent increase in cytotoxicity in both neuronal cell types. Caspase-9 and caspase-3 were activated in response to OTA treatment. Furthermore, caspase inhibitors were effective in partly counteracting OTA induced neurocytotoxicity. OTA induced apoptosis was accompanied by a loss of mitochondria membrane potential. Overall, present data indicated that OTA is neurotoxic at relatively low concentrations. OTA induced neurotoxicity seems to be, at least party, mediated by apoptosis. OTA may contribute to the pathogenesis of neurodegenerative diseases (e.g. Alzheimer’s and Parkinson’s disease) in which apoptotic processes are centrally involved. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|