Interaction of DNA with DNA binding proteins III. Infectivity of protein-complexed phage fd DNA in Escherichia coli spheroplasts |
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| Affiliation: | 1. Laboratory of Mutagenesis and DNA Damage Tolerance, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland;2. Laboratory of Molecular and Cellular Neurobiology, International Institute of Molecular and Cell Biology, ul. Ks. Trojdena 4, 02-109 Warsaw, Poland;3. Laboratory of Genomic Integrity, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA;1. Centre for Bacterial Resistance Biology, Imperial College London, London, UK;2. Department of Biology, University of Oxford, Oxford, UK;3. Parasites and Microbes Programme, Wellcome Sanger Institute, Cambridge, UK |
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| Abstract: | Complex formation of circular, single-stranded phage fd DNA with Escherichia coli DNA binding protein HD or phage fd gene 5 protein keeps infection of E. coli spheroplasts at the level of free phage DNA, whereas complexes of this DNA with E. coli DNA unwinding protein show a strongly reduced efficiency of transfection. Displacement of the unwinding protein by HD protein or gene 5 protein also maintains the poor adsorption of the complexes to spheroplasts. Free E. coli DNA unwinding protein and residual amounts of this protein bound to the DNA may interfere with the adsorption and the uptake of the phage genome. |
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