Cloning and expression of the tubulin genes in barley |
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Authors: | Radchuk Volodymyr Sreenivasulu Nese Blume Yaroslav Weschke Winfriede |
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Institution: | Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung, D-06466 Gatersleben, Germany. radchukv@ipk-gatersleben.deradchukv@ipk-gatersleben.de |
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Abstract: | Tubulins are encoded by small gene families in plants. Based on the barley EST collection, cDNAs for alpha-, beta-, and gamma-tubulins were selected. Five genes for alpha-tubulin, eight newly identified beta-tubulin sequences and one gamma-tubulin gene were found to be expressed in barley. In silico analysis of relative abundance of the distinct tubulin sequences among ESTs derived from different libraries revealed that the various tubulin genes differed in their level of expression, and to some extent were tissue specific. |
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Keywords: | Barley Tubulins Expression Development |
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