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Random sequencing of cDNA libraries reveals a variety of expressed genes in cultured cells of rice (Oryza sativa L.) |
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| Authors: | Hirofumi Uchimiya Shin-ichiro Kidou Tetsuo Shimazaki Satoshi Aotsuka Susumu Takamatsu Ritsuko Nishi Hiroshi Hashimoto Yuko Matsubayashi Naomi Kidou Masaaki Umeda Atsushi Kato |
| Affiliation: | Institute of Applied Microbiology, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan;Department of Botany, Hokkaido University, Sapporo 060, Japan;POLA Corporation, Plant Bioscience Laboratory, Totsuka-ku, Yokohama 244, Japan;Fukui Agricultural Experiment Station, Ryo, Fukui City 910, Japan;NISSHINBO INDUSTRIES, INC., Tokyo Research Center, 1-18-1 Nishiarai Sakaecho, Adachi-ku, Tokyo 123, Japan |
| Abstract: | Large scale sequencing of randomly selected cDNA clones was carried out to investigate the feasibility of this method for isolating plant genes. cDNA libraries were made using mRNA prepared from suspension-cultured cells of rice (Oryza sativa L.). Partial nucleotide sequences of 830 individual cDNA clones have been determined and compared with the GenBank database. Approximately 8% of the cDNA clones could be identified as particular genes. This method provides the opportunity to isolate large numbers of plant genes. |
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