Nucleotide diversity of a genomic sequence similar to <Emphasis Type="Italic">SHATTERPROOF</Emphasis> (<Emphasis Type="Italic">PvSHP1</Emphasis>) in domesticated and wild common bean (<Emphasis Type="Italic">Phaseolus vulgaris</Emphasis> L.) |
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Authors: | L Nanni E Bitocchi E Bellucci M Rossi D Rau G Attene P Gepts R Papa |
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Institution: | 1.Dipartimento di Scienze Agrarie, Alimentari ed Ambientali,Università Politecnica delle Marche,Ancona,Italy;2.Dipartimento di Scienze Agronomiche e Genetica Vegetale Agraria,Università degli Studi di Sassari,Sassari,Italy;3.Department of Plant Sciences/MS1,Section of Crop and Ecosystem Sciences,Davis,USA;4.Cereal Research Centre, Agricultural Research Council (CRA-CER),Foggia,Italy |
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Abstract: | Evolutionary studies in plant and animal breeding are aimed at understanding the structure and organization of genetic variations
of species. We have identified and characterized a genomic sequence in Phaseolus vulgaris of 1,200 bp (PvSHP1) that is homologous to SHATTERPROOF-1 (SHP1), a gene involved in control of fruit shattering in Arabidopsis thaliana. The PvSHP1 fragment was mapped to chromosome Pv06 in P. vulgaris and is linked to the flower and seed color gene V. Amplification of the PvSHP1 sequence from the most agronomically important legume species showed a high degree of interspecies diversity in the introns
within the Phaseoleae, while the coding region was conserved across distant taxa. Sequencing of the PvSHP1 sequence in a sample of 91 wild and domesticated genotypes that span the geographic distribution of this species in the centers
of origin showed that PvSHP1 is highly polymorphic and, therefore, particularly useful to further investigate the origin and domestication history of
P. vulgaris. Our data confirm the gene pool structure seen in P. vulgaris along with independent domestication processes in the Andes and Mesoamerica; they provide additional evidence for a single
domestication event in Mesoamerica. Moreover, our results support the Mesoamerican origin of this species. Finally, we have
developed three indel-spanning markers that will be very useful for bean germplasm characterization, and particularly to trace
the distribution of the domesticated Andean and Mesoamerican gene pools. |
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