The influence of oligonucleotide-effector on the selectivity of sequence specific modification of 16 S rRNA |
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| Authors: | M A Zenkova O S Fedorova A S Levina S V Mamaev G G Karpova |
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| Affiliation: | Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of USSR Academy of Sciences. |
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| Abstract: | The influence of duplex stabilizing oligonucleotide-effector (oligonucleotide, carrying N-(2-hydroxyethyl)phenazinium residues on both ends), on selectivity of site-directed modification of E. coli 16 S rRNA (1542 nucleotides in length) under the conditions of its secondary structure stability was studied. The constant of cooperative binding of the reagent and the oligonucleotide-effector with 16 S rRNA was determined. The accuracy of modification was shown to double in the presence of 50 microM effector at 5 microM concentration of the reagent. |
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