Mild conditions for releasing mono and bis-biotinylated macromolecules from immobilized streptavidin |
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Authors: | Nguyen Giang H Milea Jaqueline S Rai Anurag Smith Cassandra L |
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Institution: | Molecular Biology Research Laboratory, and Departments of Biomedical Engineering, Biology and Pharmacology, 36 Cummington Street, Boston, MA 02215, USA. |
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Abstract: | The high affinity (kd= approximately 10(-15)M) of streptavidin and avidin for biotin is key to a large number of biological applications and is essentially irreversible unless the complex is exposed to harsh conditions (e.g. heat (100 degrees C for 10 min)), detergents, and/or denaturants which damage macromolecules. Thus, high binding affinity becomes a disadvantage when a biotinylated target must be released for further processing. This work describes relatively mild conditions that release biotin and mono- and bis-biotinylated macromolecules from immobilized streptavidin on monodispersed magnetic beads. |
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