Mini-chromosomes: plasmids which carry the E. coli replication origin. |
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| Authors: | W Messer H E N Bergmans M Meijer J E Womack F G Hansen and K von Meyenburg |
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| Institution: | (1) Abt. Trautner, Max-Planck-Institut für molekulare Genetik, D-1000 Berlin 33;(2) University Institute of Microbiology, Copenhagen, Denmark;(3) Present address: Department of Biochemistry, Texas A & M University, College Station, Texas, U.S.A.;(4) MPI für Molekulare Genetik, Ihnestraße 63-73, D-1000 Berlin 33 |
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| Abstract: | Summary We have isolated plasmids by linking the 5.9 MD EcoRI fragment of E. coli that carries the origin of replication to an EcoRI fragment that carries an ampicillin resistance determinant, but lacks an origin of replication. 3 plasmids of this type, pOC1, pOC2, and pOC3, are described in detail in this report. Although the plasmids have some adverse effect on the growth properties of the host strain, their existence shows that two functioning chromosomal origins can coexist in one cell.Deletions generated from this type of plasmids allow an allocation of the origin of replication of E. coli within a DNA segment less than 0.4 MD in size. |
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