Protein Synthesis in Isolated Plastids during Chloroplast Development in Wheat |
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Authors: | Obokata Junichi |
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Affiliation: | Department of Botany, Faculty of Science, Hokkaido University Sapporo 060, Japan 1Research Reactor Institute, Kyoto University Present address: Kumatori-cho Sennan-gun, Osaka 590-04, Japan |
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Abstract: | Light-driven protein synthesis in isolated plastids was studiedduring the greening of etiolated wheat (Triticum aestivum L.)seedlings. The process was divided into five phases (I to V)according to the recovery of plastids from the leaf tissue.The activity was not detected in the etioplasts, but rapidlyincreased to the maximum level in phase I and remained at thislevel through phase II. During the transition from phase IIto III, the activity rapidly decreased to one-third and thencontinued to decrease slowly. The plastid polypeptides synthesizedduring the greening were analyzed by SDS-polyacrylamide gelelectrophoresis. In phase I, membrane polypeptides having molecularweights of about 21k were synthesized, while 23 k membrane polypeptidewas synthesized in phases III, IV and V. Synthesis of solublepolypeptides of 5060 k and membrane polypeptides of 15k and 3035 k was active in phases I and II, but decreasedbetween phases II and III. (Received October 31, 1983; Accepted May 14, 1984) |
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