High-level bacterial expression and purification of apicomplexan micronemal proteins for structural studies |
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| Authors: | Saouros S Blumenschein T M A Sawmynaden K Marchant J Koutroukides T Liu B Simpson P Carpenter E P Matthews S J |
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| Affiliation: | Department of Biological Sciences, Division of Molecular Biosciences, Centre for Structural Biology, Imperial College London, South Kensington, London SW7 2AZ, UK. |
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| Abstract: | The cysteine-rich N-terminal domain of the micronemal adhesive protein MIC1 (MIC1-NT) from Toxoplasma gondii was cloned, expressed in Escherichia coli and purified. MIC1-NT is amenable to structural studies as shown by preliminary NMR and X-ray analysis. Positive results with two further micronemal proteins indicate that our strategy has wider application. |
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