光肩星天牛体内纤维素酶活性及其与寄主选择的关系（英文）

为了揭示光肩星天牛Anoplophora glabripennis Motschulsky纤维素酶与寄主选择的关系, 以4种不同寄主树种（新疆杨Populus alba var. pyramidalis Bunge、箭杆杨Populus nigra var. thevestina (Dode) Bean、合作杨Populus simonii × Populus pyramidalis cv. opera Hsu和漳河柳Salix matsudana f. lobato-glandulosa Faug et Liu来源的光肩星天牛幼虫, 和以取食5种不同树种（臭椿Ailanthus altissima、毛白杨Populus tomentosa、合作杨、旱柳Salix matsudana Koidz.和复叶槭Acer negundo Linn.）的光肩星天牛成虫为实验对象, 测定其纤维素酶活性。结果表明: 不同寄主来源的光肩星天牛幼虫内切-β-1,4-葡聚糖酶活性在1.36~2.71 μmol葡萄糖/ (g FW·h)之间, β-葡萄糖苷酶活性在2.57~4.86 μmol葡萄糖/(g FW·h)之间；取食不同树种的光肩星天牛成虫内切-β-1,4-葡聚糖酶活性在4.08~9.27 μmol葡萄糖/(g FW·h)之间, β-葡萄糖苷酶活性在2.87~6.08 μmol葡萄糖/ (g FW·h)之间。不同寄主树种来源的光肩星天牛幼虫体内纤维素酶活性无显著性差异, 取食与否以及取食树种的不同对光肩星天牛成虫纤维素酶活有较大影响。

Cellulase activity and its relationship with host selection in the Asian longhorned beetle,Anoplophora glabripennis(Coleoptera:Cerambycidae)

To investigate the relationship between cellulase activity and host selection,Anoplophora glabripennis(Motsch.)larvae from four different host trees(Populus alba var.pyramidalis Bunge,Populus nigra var.thevestina(Dode)Bean,Populus simonii×Populus pyramidalis cv.opera Hsu and Salix matsudana f.Iobato-glandulosa Fang et Liu)and adults fed on five different tree species were selected.The enzyme activities of endoglucanase and β-glucosidase in the intestines of the insects were measured.The results showed that in the larvae,the activity of endoglucanase ranged from 1.36 to 2.71 μmol glucose/(g adults fed on different tree species,the activity of endoglucanase ranged from 4.08 to 9.27 μmol glucose/There are no significant differences in cellulase activities among the larvae from different host trees.However,the tree species which the adults fed upon has great effect on the activity of cellulase in the adults.