Altered expression and glycosylation of plasma proteins in rheumatoid arthritis |
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Authors: | Sunil K Raghav Bhawna Gupta Charu Agrawal Ashish Saroha Rakha H Das Ved P Chaturvedi Hasi R Das |
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Institution: | (1) Institute of Genomics & Integrative Biology, Delhi University Campus, Mall Road, Delhi, 110 007, India;(2) Department of Rheumatology, R&R, Army Hospital, N. Delhi, 110 010, India |
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Abstract: | Altered glycosylation of plasma proteins has been directly implicated in the pathogenesis of rheumatoid arthritis (RA). The
present study investigated the changes in the Concanavalin-A (Con-A)-bound plasma proteins in the RA patients in comparison to that of the healthy controls. Two proteins (MW ∼32 kDa
and ∼62 kDa) showed an alteration in expression while an altered monosaccharide profile (high mannose) was observed in the
∼62 kDa protein in the samples collected from RA patients. The 2-dimensional polyacrylamide gel electrophoresis analysis of
the Con-A-bound plasma samples showed a large number of protein spots, a few of which were differentially expressed in the
RA patients. Some unidentified proteins were detected in the RA patients which were absent in the control samples. The present
study, therefore, enunciates the role of carbohydrates as well as that of the acute phase response in the disease pathogenesis. |
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Keywords: | Rheumatoid arthritis Concanavalin A Altered glycosylation Plasma proteins 2-dimensional gel electrophoresis |
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