| 外源性p53诱导肝癌细胞Wnt通路抑制因子Dickkopf-1的表达 |
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| 引用本文： | 腊蕾,饶进军,吴曙光. 外源性p53诱导肝癌细胞Wnt通路抑制因子Dickkopf-1的表达[J]. 生命科学研究, 2004, 8(1): 63-66 |
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| 作者姓名： | 腊蕾 饶进军 吴曙光 |
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| 作者单位： | 1. 第一军医大学药物研究所,中国,广东,广州,510515
2. 第一军医大学,分校药理教研室,中国,广东,广州,510315 |
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| 摘 要： | 研究p53对Wnt通路抑制抑制因子Dickkopf-1(DKK-1)表达的调节作用,将携带p53基因的复制缺陷型腺病毒载体(Adp53)导入到p53缺失的人肝癌细胞株Hep3B中，以RT-PCR技术检测p53对DKK-1表达的调节作用．检测结果表明DKK-1 mRNA水平在转染p53 20h后即有明显升高，其中以32h达最高水平，随后逐渐降低,量效关系研究表明在转染剂量为0、5、5、50pfu／cell的Adp53时DKK-1mRNA表达均有显著增高，尤以50pfu／cell时表达水平最高。提示p53能明显诱导Wnt通路抑制因子DKK-1的mRNA表达。
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| 关 键 词： | 肝癌细胞 Wnt通路抑制因子 Dickkopf-1 DKK-1 表达 p53基因 调节作用 肿瘤 |
| 文章编号： | 1007-7847(2004)01-0063-04 |
| 修稿时间： | 2003-10-27 |
Ectogenetic p53 May Promote mRNA Expression of Wnt Signaling Inhibitor Dickkopf-1 in Hep3B Hepatoma Cells |
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| LA Lei,RAO Jin-jun,WU Shu-guang. Ectogenetic p53 May Promote mRNA Expression of Wnt Signaling Inhibitor Dickkopf-1 in Hep3B Hepatoma Cells[J]. Life Science Research, 2004, 8(1): 63-66 |
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| Authors: | LA Lei RAO Jin-jun WU Shu-guang |
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| Abstract: | To examine whether p53 has any regulatory effects on the expression of Dickkopf-1 ( Dkk-1), a Wnt pathway inhibitor, Hep3B hepatoma cells, were transfected with areplication-defective adenovirus encoding p53(Adp53), and then dickkopf-1 (DKK-1) mRNA expression was detected by RT-PCR. Dkk-1 mRNA expression in Hep3B cells was initially potentiated by transfection with Adp53 after 20 h. A peak expression was seen at 32 h and the expression declined afterwards. Dose-effect studies revealed that Adp53 in the range of 0. 5, 5 and 50 pfu/cell could promote the expression of DKK-1 mRNA, with the highest expression at the dose of 50 pfu/cell. Ectogenetic p53 promoted mRNA expression of Wnt signaling pathway inhibitor dickkopf-1 in Hep3B hepatoma cells. |
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| Keywords: | Adp53 dickkopf-1 Wnt signaling pathway |
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