Comparison of AP-PCR methods,ribotyping (PCR-ribotyping) and pulsed-field electrophoresis (PFGE) for strains of Clostridium difficile producing toxin B and not producing toxin A |
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Authors: | Pituch Hanna van Belkum Alex Obuch-Woszczatyński Piotr Meisel-Mikołajczyk Felicja Łuczak Miroslaw |
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Institution: | Katedra i Zak?ad Mikrobiologii Lekarskiej AM w Warszawie. |
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Abstract: | In this study were used AP-PCR, PCR-ribotyping and pulsed-field elecrophoresis (PFGE) for comparative study of toxin A-negative/toxin B-posi-tive Clostridium difficile strains with deletion in toxin A gen. We investigated nine unrelated clinical strains, isolated from different units and different time from patients suffering to antibiotic associated diarrhea (AAD). We found that toxin A-negative/toxin B-positive C. difficile strains isolated in Poland belonging to a single genotype A, are being similar to the Japanese strains. |
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