Inhibition by epigallocatechin gallate of CoCl2-induced apoptosis in rat PC12 cells |
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Authors: | Jung Ji-Yeon Mo Hyun-Chul Yang Kyu-Ho Jeong Yeon-Jin Yoo Hyeon-Gyeong Choi Nam-Ki Oh Won-Mann Oh Hee-Kyun Kim Sun-Hun Lee Jin-Ha Kim Hyun-Jin Kim Won-Jae |
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Affiliation: | Dental Science Research Institute, School of Dentistry, 2nd Stage of Brain Korea 21 for School of Dentistry, Chonnam National University, Gwang Ju 500-757, South Korea. |
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Abstract: | Epigallocatechin-3-gallate (EGCG) is a major constituent of green tea polyphenols. This study was aimed to investigate the possible mechanisms of EGCG-mediated inhibition against apoptosis in rat pheochromocytoma PC12 cells by exposure to CoCl(2). Exposure to CoCl(2) caused the generation of ROS and induced cell death with appearance of apoptotic morphology and DNA fragmentation. However, EGCG rescued the loss of viability in the cells exposed to CoCl(2) and led the reduction of DNA fragmentation and sub-G(1) fraction of cell cycle. Also, EGCG attenuated the CoCl(2)-induced disruption of mitochondrial membrane potential (DeltaPsim), release of cytochrome c from the mitochondria to cytosol and abolished the CoCl(2)-stimulated activities of the caspase cascades, caspase-9 and caspase-3. In addition, EGCG ameliorated the increase in the Bax to Bcl-2 ratio, a marker of apoptosis proceeding, induced by CoCl(2) treatment. Taken together, the present results suggest that EGCG inhibit the CoCl(2)-induced apoptosis of PC12 cells through the mitochondria-mediated apoptosis pathway involved in modulating the Bcl-2 family. |
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Keywords: | Eigallocatechin-3-gallate CoCl2 Rat PC12 cells Apoptosis Reactive oxygen species Mitochondria Bcl-2 family |
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