Cloning, expression and purification of SmpB from Mycobacterium tuberculosis |
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| Authors: | Kovács L Megyeri Klára Juhász Anna Zaja Anikó Miczák A |
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| Institution: | Department of Medical Microbiology and Immunobiology, University of Szeged, PO Box 427, H-6701 Szeged, Szeged, Hungary. |
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| Abstract: | SmpB, a small tmRNA binding protein, is essential for trans-translation. 6His and FLAG tagged SmpB was cloned from Mycobacterium tuberculosis H37Rv. It was expressed in Escherichia coli using the T7 promoter-polymerase system. Anti-FLAG M2 agarose was used for its purification. Mycobacterial SmpB copurifies with other proteins. We identified elongation factor EF-Tu in the purified SmpB preparations. |
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