Frequency of telomere repeat units in the Drosophila miranda genome |
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Authors: | M Steinemann U Nauber |
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Institution: | (1) Max-Planck-Institut für Biologie, Spemannstr. 34, D-7400 Tübingen;(2) Present address: Abt. Genetik und Entwicklungsbiologie, Universität Salzburg, Erzabt-Klotzstr. 11, A-5020 Salzburg, Austria |
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Abstract: | Cloned DNA fragments of Drosophila miranda which label all chromosome ends show a basic tandem repeat unit of 4.4 kb. The D. miranda telomere specific tandem repeats do not cross-hybridize with genomic D. melanogaster DNA which itself contains telomere repeat units of 3 kb. For a more detailed analysis of the functional criteria of telomere specific sequences we determined the repetition frequency of the tandem repeat units. As a low estimate we found a repetition frequency of 20 for female D. miranda DNA. This is on average equivalent to 2 telomere repeat units per chromosome end in the female D. miranda karyotype. However, a variable number of tandem repeat units per chromosome end would describe more closely the obtained differences in the labeling intensity between the individual chromosomes (X1L-5). For the D. miranda male DNA we determined a repetition frequency of 90. The frequency difference of 70 copies between male and female DNA must be due to the Y-chromosome. |
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