Analysis of helper-T-cell function by acetylcholine receptor-reactive cell lines of defined AChR-subunit specificity |
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Authors: | K A Krolick O E Urso |
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Institution: | 1. Baylor Scott & White Health, Dallas, Texas;2. University of Liege Hospital, GIGA Cardiovascular Sciences, Department of Cardiology, CHU Sart Tilman, Liege, Belgium;3. Gruppo Villa Maria Care and Research, Maria Cecilia Hospital, Cotignola, and Anthea Hospital, Bari, Italy;1. Department of Pharmaceutical Sciences, College of Pharmacy, USA;2. Skeletal Biology Laboratory, School of Biological and Population Health Sciences, Oregon State University, Corvallis, OR 97331, USA;3. Department of Integrative Biosciences, Oregon Health & Science University, Portland, OR 97201, USA |
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Abstract: | A small panel of cloned acetylcholine receptor (AChR)-reactive helper T cells were examined for their ability to proliferate when stimulated with AChR or purified AChR subunits. It was observed that all T-cell lines preferentially responded to the AChR alpha subunit, but that some also were stimulated by other subunits as well. This was interpreted as indicating that the alpha subunit plays a major role in anti-AChR T-cell responses, but that considerable potential cross-reactivity exists among the subunits recognized by T cells. Furthermore, a high level of "microheterogeneity" in the T-cell-specificity repertoire was suggested by the fact that even this small panel of cloned lines expressed several patterns of subunit reactivity. Finally, all cloned T-cell lines examined were found to be capable of functioning as regulatory helpers in vitro by providing the necessary signals to AChR-responsive B cells, resulting in anti-AChR antibody production. |
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