Transformed roots of Crepis capillaries — a sensitive system for the evaluation of the clastogenicity of abiotic agents |
| |
Authors: | Jolanta Juchimiuk Jolanta Maluszynska |
| |
Affiliation: | Department of Plant Anatomy and Cytology, University of Silesia, Jagiellonska 28, 40-032 Katowice, Poland |
| |
Abstract: | The presence of a large number of pollutants, including mutagenic agents in the environment is a problem of a major concern. Rapid progress in plant biotechnology, especially in the development of cell transformation methods, including the production of transformed roots – ‘hairy roots’ – has opened new possibilities to use transformed root cultures in plant bioassays for the evaluation mutagenic effects of different agents. We have used Crepis capillaris hairy roots for evaluation of cytogenetic effects of mutagenic treatment. Effects of maleic acid hydrazide (MH) and X-ray treatment were analysed in chromosomal aberration, sister chromatid exchange (SCE) and TUNEL tests. Comparison of cytogenetic effects in hairy roots and roots of seedlings showed a much higher sensitivity of hairy roots, which makes them convenient material for monitoring DNA damage after mutagenic treatment. |
| |
Keywords: | Hairy roots Crepis capillaris In vitro culture Plant bioassay Genotoxicity Chromosomal aberrations |
本文献已被 ScienceDirect 等数据库收录! |
|