Glutamate- and GABA-mediated neuron–satellite cell interaction in nodose ganglia as revealed by intracellular calcium imaging |
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Authors: | Yuko Shoji Misuzu Yamaguchi-Yamada Yoshio Yamamoto |
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Institution: | (1) Laboratory of Veterinary Biochemistry and Cell Biology, Faculty of Agriculture, Iwate University, Morioka Iwate, 020-8550, Japan;(2) Laboratory of Veterinary Biochemistry and Cell Biology, Faculty of Agriculture, Iwate University, 18-8, Ueda 3-chome, Morioka Iwate, 020-8550, Japan; |
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Abstract: | In the sensory ganglia, neurons are devoid of synaptic contacts, and ganglion neurons surrounded by one of glial cells, satellite
cells. Recent studies suggest that neurons and satellite cells interact through neurotransmitters. In the present study, intracellular
Ca2+ (Ca2+]i) dynamics of neurons and satellite cells from one of viscerosensory ganglia, nodose ganglion (NG), were investigated by stimulation
with glutamate and its agonist and/or the antagonist of the GABAA receptor bicuculline. In the specimens containing neurons with satellite cells, glutamate and a metabotropic glutamate receptor
(mGluR) agonist t-ACPD evoked Ca2+]i increases in both neurons and surrounding satellite cells. Moreover, bicuculline also induced Ca2+]i increases in neurons and satellite cells. However, in the isolated neurons, bicuculline did not cause an increase in Ca2+]i, suggesting that satellite cells are equipped with the ability to release GABA. In the neurons associated with satellite
cells, the delay time until the onset of a response was shorter in the case of glutamate stimulation with bicuculline than
that without bicuculline (107.3 ± 93.4 vs. 231.8 ± 97.0 s, p < 0.01). Furthermore, immunoreactivities for glutamate transporter, GLAST, and GABA transporter, GAT-3, were observed in
both neurons and satellite cells of NG. In conclusion, the levels of Ca2+]i of NG neurons and surrounding satellite cells are increased by glutamate through at least mGluRs, and endogenous GABA modulates
these responses; GABA inhibition is dependent on a close association between neurons and satellite cells. Such neuron–glia
interaction in the nodose ganglion may regulate sensory information from visceral organs. |
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