SED88: a Pascal program for the analysis of sedimentation equilibrium data |
| |
| Authors: | Reid, P. M. Wilkinson, A. E. Leung, K. C. Jones, M. N. |
| |
| Affiliation: | Department of Biochemistry and Molecular Biology, University of Manchester Manchester MI3 9PT
1Department of Electrical Engineering and Electronics, University of Manchester Institute of Science and Technology PO Box 88, Manchester M60 IQD, UK |
| |
| Abstract: | Analytical ultracentrifugation is commonly used for the determinationof molecular weights (sedimentation equilibrium) and sedimentationcoefficients (sedimentation rate) of biological macromoleculesin solution. A Turbo Pascal program for the analysis of sedimentationequilibrium centrifugation data produced by absorbance opticalsystems is described. The user may enter data from a scan ofabsorbance versus distance from the centre of rotation, viaa graphics tablet (or ASCII file). This is subsequently manipulatedto yield an apparent weight average molecular weight for thegiven sample. Plots of In (absorbance) versus (radius2) mayalso be produced. The method described uses readily availablecomputational equipment requiring only a graphics tablet inaddition to an IBM PC compatible computer. This technique andthe software developed have been used to investigate the molecularweight range of two International Humic Substances Society (IHSS)reference samples from the Suwannee River. Received on October 7, 1988; accepted on December 12, 1988 |
| |
| Keywords: | |
| 本文献已被 Oxford 等数据库收录！ |
|
